اثر عصاره آبی و هیدروالکلی ساقه هوایی گیاه شوید بر تشنجات ناشی از تزریق پنتیلن‌تترازول در موش سوری نر

  Effects of the Aqueous and Alcoholic Extracts of Anethum Graveolens L (dill) on Pentylenetetrazol-induced Seizures in Male Mice     M. Mohammadzadeh [1] , A. Pajhhan1, S.J. Mirnajafizadeh [2] , M.H. Rakhshani [3]     Received: 13/10/2010 Sent for Revision: 13/11/2010 Received Revised Manuscript: 05/04/2011 Accepted: 26/04/2011     Background and Objectives : In this study, the effect of Anethum graveolens L. extract on pentylenetetrazol- (PTZ) induced seizures was investigated.   Materials and Methods: In this experimental study, 110 male mice were included. Firstly, the maximum non-lethal doses for aqueous and alcoholic extract of Anethum graveolens L. were determined. Three doses of aqueous (250, 400, and 500 mg/kg) and four doses of alcoholic extracts (250, 1000, 2500, and 3500 mg/kg) were intraperitoneally injected 30 minutes before PTZ injection. All doses were lower than the non-lethal dose Seizure stage 1 latency (S1L), seizure stage 5 latency (S5L) and dead time latency (DTL) were recorded after PTZ injection in both control and treatment groups. One-way and two way ANOVA were applied for comparing the results of this study.   Results: Aqueous extract of Anethum graveolens L . at 500 mg/kg increased S1L, S5L and DTL significantly (p<0.01, p<0.05 and p<0.01 respectively). Concentration of 400 mg/kg was able to markedly increase only S1L and DTL (p<0.05 and p<0.01 respectively) and Concentration or 250 mg/kg had no any effects.   Injection of the alcoholic extract at concentrations of 1000, 2500 and 3500 mg increased S1L, S5L and DTL significantly. However, concentration of 250 mg/kg significantly increased only S1L and DTL (p<0.05 and p<0.01 respectively). There was no significant between aqueous and alcoholic extract except in DTL.   Conclusion: The results indicated that the aqueous and alcoholic extractions of Anethum graveolens L. possibly have protective effects on nervous system and also decrease seizure induced by PTZ.   Key words: Seizure, Epilepsy, Pentylenetetrazol, Anethum graveolens, Mice     Funding: This research was funded by Sabzevar University of Medical Sciences.   Conflict of interest: None declared.   Ethical approval: The Ethics Committee of Sabzevar University of Medical Sciences approved the study.     How to cite this article : Mohammadzadeh M, Pajhhan A, Mirnajafizadeh S.J, Rakhshani M.H. Effects of the Aqueous and Alcoholic Extracts of Anethum Graveolens L (dill) on Pentylenetetrazol-induced Seizures in Male Mice. J Rafsanjan Univ Med scie 2012 11(1): 47-56. [Farsi]       [1] - Assistant Prof., Dept. of Physiology, Sabzevar University of Medical Sciences, Sabzevar, Iran   (Corresponding Author) (0571) 4446070, Fax:(0571) 4445648, E-mail: [email protected]   [2] - Prof., Dept. of Physiology, Tarbiat Modarres University, Tehranl, Iran   [3] - Academic Member, Dept. of Statistics, Sabzevar University of Medical Sciences, Sabzevar, Iran